Western blot analysis of extracts from HeLa cells transfected with 20 nM control siRNA #6201, or 5, 20 nM pool p38 MAP Kianse siRNA, using p38 MAP Kinase Antibody #9212 (upper), p44/42 MAP Kinase Antibody #9102 (middle) and SAPK/JNK (56G8) Rabbit mAb #9258 (lower) in combination with ATP-Citrate Lyase Antibody #4332. The p38 MAP Kinase Antibody confirms silencing of p38 MAP Kinase expression, and ATP-Citrate Lyase Antibody is used to control for loading and specificity of pool p38 MAP Kinase siRNA. The experiment also demonstrates specifically that the pool p38 MAPK siRNA does not interfere with the expression of p44/42 MAPK and SAPK/JNK.